Actinomycetes are microorganisms native to the sea and can be found on the surface of seawater, the seabed, in sediments, coral reefs, and in soil. Actinomycetes are prokaryotic, like bacteria, but possess mycelium and are classified as microbes with the potential to produce secondary metabolites. Actinomycetes form symbiotic relationships with sponges on the outside of the body (extracellular exosymbiosis), inside the sponge (extracellular endosymbiosis), in the cytoplasm (intracellular symbiosis), and in the cell nucleus (intranuclear symbiosis). Actinomycetes are capable of producing bioactive compounds from antibiotics (70%), fungi (20%), and bacteria (10%). This type of microbe has been widely used in the pharmaceutical, animal feed, fisheries, and other industries. Commercial products that utilize actinomycete secondary metabolites include tetracycline, erythromycin, vancomycin, and streptomycin.
Sponges are marine invertebrates that possess more than 20 categories of bioactive compounds, including antibiotics, antivirals, anti-inflammatories, anticancer, antileukemic, insecticidal, antihelminthic, and cytotoxins, making them attractive for development. Nutritionally, sponges are more suitable as microbial niches than nutrient-poor seawater. The interaction between bacteria and sponges is called mutualistic symbiosis, producing bioactive compounds. Sponges with high microbial abundance are called High-Microbial-Abundance (HMA), while sponges with low microbial abundance are called Low-Microbial-Abundance (LMA). The microbes that symbiotically interact with sponges, known as secondary metabolite producers, are members of the Actinobacteria group.